• 专利附录
  • 专利说明
  • 权利要求
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  • 优先权
    • 专利摘要:
    The present invention provides a process method for producing erythritol from wheat starch, and belongs to the technical field of deep processing of the wheat. As wheat starch is graded and separated on the wheat gluten line, the quality of starch particles obtained is guaranteed by controlling the separation ratio of starch; by controlling the content of soluble solids in the washing water, the quality of starch slurry can be improved, and the fermentation and extraction of erythritol are facilitated; and by retaining some nutrients of wheat, the addition of yeast extract for an erythritol fermentation medium can be reduced. High-quality wheat starch separated from the wheat gluten line is directly used for erythritol fermentation, which can not only improve the added value of the wheat starch, but also greatly reduce the raw material cost of erythritol, and has very good economic benefits and good practical application value.
    公开号:NL2028015A
    申请号:NL2028015
    申请日:2021-04-20
    公开日:2021-10-27
    发明作者:Tian Yanjun；Han Mo；Liu Liping；Liu Jianjun；Zhang Jiaxiang；Zhao Xiangying
    申请人:Shandong Food Ferment Industry Res & Design Institute；
    IPC主号:
    专利说明:

    i
    PROCESS METHOD FOR PRODUCING ERYTHRITOL FROM WHEAT STARCH Field of the Invention The present invention belongs to the technical field of deep processing of agricultural products, and specifically relates to a process for producing erythritol from wheat starch separated from the wheat gluten line.
    Background of the Invention The information disclosed in the background 1s merely intended to increase the understanding on the general background of the present invention, and should not be construed as acknowledgment or hint in any form that the information constitutes the prior art that has been known by those skilled in the art.
    Wheat gluten is a high-quality natural plant protein produced from wheat. It has a variety of unique physical and chemical properties, and has been widely used in the food industry. At present, wheat gluten has an enormous market demand and a high added value, so it will be an important direction of wheat processing to produce wheat gluten.
    Wheat contains about 12-13% of protein and 65-70% of starch, so a large amount of wheat starch is produced during the production of wheat gluten. Wheat starch is divided into A starch and B starch according to different particle sizes. At present, there are two main methods to treat wheat starch produced during wheat gluten production. One is to separate, refine and sell A starch as a commercial starch, and to produce alcohol from B starch or to concentrate and dry B starch as a feed. The other is to use all the starch for alcohol fermentation. The production of commercial starch requires washing, dehydration and drying of the A starch, so its production cost is higher than that of corn starch, and has little market competitiveness. If all the starches are used for alcohol fermentation, the added value of the product is low. The quality of the wheat A starch is relatively good. If the separated A starch slurry is directly used for the bio-transformation production of products with high added values, not only can the production cost of target products be greatly reduced, but also the added value of the wheat starch can be improved.
    Erythritol is a new sugar alcohol sweetener. It has the characteristics of good crystallinity, low moisture absorption, low calorie, coordinated sweet taste, no dental decay and the like. Therefore, it has a wide range of use in the food industry and has a high product added value. At present, erythritol is produced from glucose by fermentation with a yeast and then purification, concentration and crystallization of the fermentation liquid. When erythritol is produced from starch, the starch needs first to be hydrolyzed to glucose and then to use glucose for erythritol fermentation. The inventors have found that, because of the characteristics of the erythritol production, the process of erythritol production requires relatively high quality of raw starch.
    Summary of the Invention Aiming at the problem of low-value use of wheat starch produced during the production of wheat gluten, the present invention provides a process for producing erythritol from wheat starch separated from the wheat gluten line. Specifically, some starch is obtained by centrifugal separation of wheat starch slurry produced during the production of wheat gluten, and then liquefied and saccharified for erythritol production. With the above production process, the production cost of erythritol is reduced, and the added value of wheat starch is increased; therefore, the process has good actual application value. In order to achieve the above technical objectives, the technical solutions of the present invention are as follows: The first aspect of the present invention provides a process method for producing erythritol from wheat starch separated from the wheat gluten line, the process method including: performing centrifugal separation, washing and saccharification on wheat starch slurry generated during the production of wheat gluten to obtain starch saccharified liquid, preparing an erythritol fermentation medium from the starch saccharified liquid as a medium raw material, and inoculating strains for erythritol fermentation. Further, the specific method of washing is: collecting starch milk separated by centrifugation, adding water to adjust the starch milk to a suitable concentration, then washing the starch milk, and collecting bottom starch milk; Further, the saccharification method has no limitations, for example, a common “double-enzymatic method” can be used; Further, the saccharification process further includes double-enzymatic saccharification and decoloration of the saccharified liquid. The decoloration can use common methods for industrial saccharification decoloration, and activated carbon is preferably used for decoloration. The specific method of erythritol fermentation is: collecting the starch saccharified liquid as a raw material, adding accessories and nutritive salt to prepare an erythritol fermentation medium, controlling the initial glucose concentration to be 260 to 300 g/L, inoculating erythritol strains for fermentation after the medium is sterilized, finishing the fermentation after the glucose concentration is reduced to 0.1 g/L, and collecting the fermentation liquid.
    The second aspect of the present invention provides erythritol and/or wheat gluten prepared by the above-mentioned process method for producing erythritol from wheat starch separated from the wheat gluten line.
    Beneficial technical effects of the above one or more technical solutions: Wheat starch co-produced during the processing of wheat gluten is graded and separated on line, the quality of starch obtained is guaranteed by controlling the separation ratio of starch, and the content of soluble solids in the starch slurry is controlled by adjusting the degree of washing of the starch.
    The accurate control on the separation ratio and washing of starch meets the requirements of erythritol fermentation and the quality of extracted raw starch.
    Meanwhile, some nutrients of wheat are retained, which can shorten the fermentation cycle of erythritol or reduce the adding amount of nutrients of the erythritol fermentation medium.
    High-quality wheat starch separated from the wheat gluten line is directly used for erythritol fermentation production, which not only improves the processing added value of the wheat starch, but also can greatly reduce the raw material cost of erythritol, and has very good economic benefits and good practical application value.
    Brief Description of the Drawings The accompanying drawings constituting a part of the present invention are used for providing a further understanding on the present invention, and the schematic embodiments of the present invention and the descriptions thereof are used for interpreting the present invention, rather than constituting improper limitations to the present invention.
    Fig. 1 is an HPLC spectrogram of an erythritol fermentation liquid of a wheat starch saccharified liquid raw material in Embodiment 4 of the present invention; Fig. 21s an HPLC spectrogram of an erythritol fermentation liquid of a glucose raw material in Embodiment 5 of the present invention.
    Detailed Description of Embodiments It should be pointed out that the following detailed descriptions are all exemplary and aim to further illustrate the present invention.
    Unless otherwise specified, all technical and scientific terms used in the descriptions have the same meanings generally understood by those of ordinary skill in the art of the present invention.
    It should be noted that the terms used herein are merely for describing embodiments, but are not intended to limit exemplary embodiments according to the present invention.
    As used herein, the singular form is also intended to include the plural form unless otherwise indicated in the context. In addition, it should be understood that when the terms “contain” and/or “include” are used in the description, they are intended to indicate the presence of features, steps, operations, devices, components and/or combinations thereof. It should be appreciated that the protection scope of the present invention 1s not limited to the following specific embodiments. It should also be appreciated that the terms used in the embodiments of the present invention are to describe the specific embodiments, not to limit the protection scope of the present invention.
    In an embodiment of the present invention, a process method for producing erythritol from wheat starch separated from the wheat gluten line is provided. The process method includes: performing centrifugal separation, washing and saccharification on wheat starch slurry generated during the production of wheat gluten to obtain starch saccharified liquid, preparing an erythritol fermentation medium from the starch saccharified liquid as a medium raw material, and inoculating strains for erythritol fermentation.
    In another embodiment of the present invention, the specific method of centrifugal separation is collecting centrifugal separation starch by centrifugal separation. It is found that starch prepared in different starch separation ratios directly affects the filter rate and transparency of the subsequent saccharified liquid, which finally affect the erythritol fermentation rate and erythritol fermentation purity. The quantity of centrifugal separation starch is controlled to be 40-60% of the total starch mass of the starch slurry, for example, 40%, 45%, 50%, 53%, 55% and 60%.
    In still another embodiment of the present invention, the quantity of centrifugal separation starch can be controlled by controlling the centrifugation speed and centrifugation time, the centrifugation speed is 800-2000 rpm, for example, 800, 1000, 1200, 1500 and 2000 rpm, and the centrifugal time is 2 seconds to 1 minute, for example, 2 seconds, 10 seconds, 30 seconds and 1 minute.
    In still another embodiment of the present invention, the specific method of washing is: collecting centrifugal separation starch, adding water to adjust the concentration of the starch slurry, then washing the starch milk, and collecting the bottom starch milk.
    In still another embodiment of the present invention, the concentration of the adjusted starch slurry is 8 to 12 Bé; In still another embodiment of the present invention, the concentration of the starch milk is controlled to be 18 to 20 Bé; In still another embodiment of the present invention, the concentration of soluble solids in the starch whey 1s 0.15-0.5%. By retaining some soluble ingredients of the wheat starch slurry in the starch, the growth of the subsequent erythritol fermentation strains is facilitated, and the fermentation cycle can be shortened. By controlling the concentration of soluble solids in the starch whey, 10-20% of yeast extract in the subsequent erythritol fermentation process is 5 replaced, thereby effectively saving the cost of the raw material.
    In still another embodiment of the present invention, the saccharification method has no limitations, for example, a common “double-enzymatic method” is used.
    In still another embodiment of the present invention, the saccharification process further includes double-enzymatic saccharification and decoloration of the saccharified liquid.
    In still another embodiment of the present invention, the decoloration can use common industrial saccharification decoloration methods, and activated carbon is preferably used for decoloration.
    In still another embodiment of the present invention, the specific method of erythritol fermentation is: collecting the starch saccharified liquid as a raw material, adding accessories and nutritive salt to prepare an erythritol fermentation medium, controlling the initial glucose concentration to be 260 to 300 g/L, inoculating strains for erythritol fermentation after the medium is sterilized, finishing the fermentation after the glucose concentration is reduced to
    0.1 g/L, and collecting the fermentation liquid.
    In still another embodiment of the present invention, the strains are Yarrowia lipolytica, which is an aerobic and apathogenic dicotypic unconventional yeast and can be used for producing erythritol by glucose.
    In still another embodiment of the present invention, the process method for producing erythritol from wheat starch separated from the wheat gluten line further includes extraction and purification of the erythritol. Specifically, the extraction and purification method includes: filtering and sterilizing the collected erythritol fermentation liquid, collecting the permeated liquid, and performing concentration, crystallization, dissolution, decoloration, recrystallization, centrifugal separation and drying on the filtered clear liquid to obtain an erythritol product. The extraction and purification method can be carried out with reference to CN200710115541.9.
    In still another embodiment of the present invention, erythritol and/or wheat gluten prepared by the above-mentioned process method for producing erythritol from wheat starch separated from the wheat gluten line are provided.
    The present invention will be further explained by examples, which are not construed as limiting the present invention. It should be understood that these examples are only used to illustrate the present invention but not to limit the scope of the present invention.
    Laboratory experiment Experimental materials Wheat starch slurry: provided by a wheat gluten manufacturer, the starch slurry used in the examples was fresh separated starch slurry.
    Erythritol fermentation strain: one Yarrowia lipolytica strain.
    Experimental method (1) Separation of wheat starch: control starch grading by centrifuge speed: take fresh wheat starch slurry (mixed starch slurry containing A starch and B starch, with a concentration of 10 Bé) produced in the production process of wheat gluten, centrifuge with a centrifuge (speed: 800 to 2000 rpm, time: 2 seconds to 1 minute), then remove the supernatant and fluid slurry, collect bottom precipitate, add water for suspended washing and centrifugation till the soluble solid of the centrifuged supernatant was 0-0.5%, and collect the precipitated starch for preparing starch saccharified liquid.
    (2) Preparation of erythritol fermentation saccharified liquid: add water to the separated and washed starch to adjust the concentration of the starch slurry to be 16 to 17 Bé, add a proper amount of amylase according to the total amount of the starch for double-enzymatic saccharification (the variety and dose of the enzyme preparation were used according to the amount recommended by the enzyme preparation manufacturer), and decolor the saccharified liquid conventionally with activated carbon after the saccharification, wherein the decolored saccharified liquid can be directly used for the preparation of an erythritol fermentation medium.
    (3) Erythritol fermentation: take the above starch saccharified liquid, add yeast extract and other nutrients according to the requirements of an erythritol fermentation medium to prepare the erythritol fermentation medium, and adjust the glucose concentration to be 260 to 300 g/L. The control experiment adopted oral glucose or corn starch saccharified liquid. After the medium was sterilized, strains were inoculated for erythritol fermentation.
    Flask shaking fermentation: fill a 500 ml triangular flash with 50 ml of liquid with an inoculation amount of 5%, and culture 4-5 days under optimal culture conditions.
    Fermenter fermentation: ferment with a laboratory-scale (5-50 L) fermenter, and control the appropriate temperature, pH value and relative dissolved oxygen concentration according to the requirements during fermentation. The fermentation was finished till the residual glucose dropped to 0.1 g/L.
    (4) Erythritol extraction: filter and sterilize the collected erythritol fermentation liquid with a ceramic membrane, perform purification such as nano-filtration and ion exchange on the permeated liquid, and perform concentration, primary crystallization, dissolution, decoloration, recrystallization, centrifugal separation and drying on the purified liquid to obtain an erythritol product.
    Analytical method: Measurement of the soluble solid content of centrifugal supernatant in the laboratory: centrifuge the starch slurry to remove the supernatant and fluid slurry, add water to 1/2 of the original volume, re-suspend the precipitated starch uniformly, then delaminate by centrifugation, and hold a saccharimeter to measure the soluble matter concentration of the supernatant.
    Measurement of starch content: refer to Measurement of Starch in Food in GB5009.9-2016 Food Safety Standards.
    Measurement of glucose: use an SBA-40D biosensor analyzer to measure the content of glucose. HPLC measurement of erythritol content: purify and dilute the fermentation liquid to a proper concentration. Standard samples were prepared as 10-20 g/L solution.
    Chromatographic column: HPX-87H.
    Detector: refractive index detector.
    Mobile phase: 0.005 M sulfuric acid.
    Analysis conditions: flow rate 0.6 ml/min, column oven temperature 35°C.
    Example 1 Effect of centrifugation speed and time on separation ratio of wheat starch The ratio of high-quality starch separated from wheat starch slurry was controlled using the above-described wheat starch separation refining method by controlling the speed and time of a centrifuge, and the collected starch was washed till the soluble solid content of the supernatant was close to 0. The ratios of the separated starch obtained at different centrifugation speeds to the total starch were shown in Table 1.
    Table 1 Effect of centrifugation speed and time on separation ratio of starch Speed (rpm) Centrifugation Ratio of separated starch to time (8) total starch (%) 800 2 45 1000 2 53 1000 30 65 2000 30 85 Example 2 Effect of wheat starch separation ratio on starch saccharification and erythritol fermentation The starch separated and refined in Example 1 was prepared into starch saccharified liquid by a double-enzymatic saccharification process.
    It was found that different starch separation ratios had no obvious effects on the liquification and saccharification but affected the filter rate of the saccharified liquid.
    Especially when the separation ratio reached 80% or more, the filter rate of the saccharified liquid obviously became slow, and the transparency of the saccharified liquid decreased.
    A flask shaking fermentation experiment of erythritol fermentation was performed on the above saccharified liquid.
    The results showed (Table 2), when the starch separation ratio was 60% or less, the fermentation rate of the wheat starch saccharified liquid was similar to those of glucose and corn starch saccharified liquid, and the purity and yield of erythritol in the fermentation liquid were equivalent to those of corn starch saccharified liquid and slightly lower than those of glucose; and when the separation ratio was 80% or more, the erythritol fermentation purity was obviously reduced.
    Effect of wheat starch separation ratio on starch saccharification and erythritol fermentation Ratio of separated Filter rate of Transparency of Erythritol starch to total saccharified liquid saccharified liquid fermentation purity starch (%o) (%) (600 nm) (%) 45 Fast 99.5 93.6 53 Fast 98.6 93.1 65 Slightly slow 97.2 92.4 85 Slow 92.8 89.3
    Corn starch sugar Fast 99.3 93.3 Glucose / / 95.0 Example 3 Effect of the degree of washing on erythritol fermentation of wheat starch From the above centrifugal separation experiment results, it can be known that the centrifugal speed of 1000 rpm can realize a wheat starch separation ratio of about 50%. In this embodiment, the centrifugal speed of 1000 rpm was used for the separation of wheat starch slurry to inspect the effect of the degree of starch washing on the subsequent saccharification and erythritol fermentation.
    The experiment employed flask shaking fermentation to inspect the effect of starch washing on the erythritol fermentation rate.
    The experimental results showed (Table 3), some soluble ingredients of wheat starch slurry remaining in the starch were beneficial to shortening erythritol fermentation cycle.
    On this basis, the experiment further inspected the possibility of replacing some organic nitrogen sources with some soluble ingredients of wheat starch slurry remaining in the starch.
    The fermentation results showed that 10-20% of yeast extract can be replaced by controlling the soluble matter concentration of the starch washing supernatant, without affecting the technical indicators such as production rate and conversion rate of erythritol.
    Table 3 Effect of starch washing on erythritol fermentation Starch Soluble matter Filter rate of Residual washing times concentration of saccharified sugar g/L supernatant liquid 0 1.5 Slightly slow 10 1 0.45 Fast 0 2 0.15 Fast 0 3 0 Fast 15 Example 4 Scale-up experiment (1) Starch separation: perform continuous centrifugal separation with a disc centrifuge on 10 m of fresh wheat starch slurry with a concentration of 10 Bé produced in the production process of wheat gluten, and collect 2.3 m3 of heavy phase starch slurry with a concentration of 20 Bé, wherein the total amount of starch was 0.9 t, accounting for 50% of the total starch of the original wheat starch slurry;
    (2) Starch washing: add water to the starch slurry collected in (1) to adjust the concentration of the slurry to be 12 Bé, pump the adjusted starch milk into a cyclone for washing, and collect 2 m’ of bottom starch milk with a concentration of 20 Bé, which contained 0.83 t of starch, wherein the solid content of the clear starch slurry was 0.2%; (3) Starch saccharification: add water to the starch milk collected in (2) to adjust the concentration to be 17 Bé, adjust the pH value of the starch milk to be 6.50, add 2.0 kg of liquifying enzyme for injection liquifying, liquify at 100-120°C for 40-45 min (or refer to the specification of an enzyme preparation), then cool to 60°C, adjust the pH value of the material to be 4.40, add 2.0 kg (or refer to the specification of an enzyme preparation) of saccharification enzyme (Novozymes Dextrozyme High DX), preserve the heat at 60°C and saccharify 30 h while intermittent mixing till the DE value no longer increased. Heat the saccharified liquid to 70°C, add 8 kg of powdered activated carbon and decolor 30 min, and then perform plate and frame filtering to obtain 2.6 m of clear starch hydrolyzed and saccharified liquid, containing 316 g/L of glucose; (4) Erythritol fermentation: fill a fermenter with the clear saccharified liquid collected in (3), add water, accessories and nutritive salt to prepare an erythritol fermentation medium, control the glucose concentration to be 300 g/L, inoculate strains for erythritol fermentation after the medium was sterilized, finish the fermentation after 94 h when the glucose was consumed to be 0.06 g/L, and collect the fermentation liquid, wherein the yield of erythritol was 163 g/L; HPLC analysis on the fermentation liquid showed that the peak area of erythritol accounted for 93.5% of the total peak area (Fig. 1), (5) Extraction of erythritol: filter the erythritol fermentation liquid collected in (4) with a ceramic membrane to remove the yeast, collect the permeated liquid, and perform concentration, crystallization, dissolution, decoloration, recrystallization, centrifugal separation and drying on the filtered clear liquid to obtain an erythritol product.
    Example 5 Erythritol fermentation of glucose raw material (1) Preparation of medium: weigh crystalline glucose, add water to dissolve the crystalline glucose, add an organic nitrogen source such as yeast extract and other nutrient salt according to the requirements to prepare an erythritol fermentation medium, and control the glucose concentration to be 300 g/L.
    i (2) Erythritol fermentation: inoculate strains for erythritol fermentation after the medium was sterilized, finish the fermentation after 98 h when the glucose was consumed to be 0.02 g/L, and collect the fermentation liquid, wherein the yield of erythritol was 165 g/L. HPLC analysis on the fermentation liquid showed that the peak area of erythritol accounted for 95% of the total peak area (Fig. 2); (3) Extraction of erythritol: filter the collected erythritol fermentation liquid with a ceramic membrane to remove the yeast, collect the permeated liquid, and perform concentration, crystallization, dissolution, decoloration, recrystallization, centrifugal separation and drying on the filtered clear liquid to obtain an erythritol product.
    It should be noted that the above examples are only used to illustrate the technical solutions of the present invention rather than limiting them. Although the present invention is described in detail with reference to the given examples, those of ordinary skill in the art could modify or equivalently replace the technical solutions of the present invention as required without departing from the spirit and scope of the technical solutions of the present invention.
    权利要求:
    Claims (13)
    [1]
    A method for preparing erythritol from wheat starch separated from the wheat gluten production line, the method comprising: centrifugal separation, washing and saccharification, and generating the starch sugar solution, and using it as a medium raw material for erythritol fermentation.
    [2]
    The method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 1, wherein the method step of centrifugal separation comprises: collecting starch separated by centrifugation.
    [3]
    A method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 2, wherein the amount of separation starch is controlled at 40-60% of the total starch mass of the starch slurry
    [4]
    The method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 1, 2 or 3, wherein the method step of washing comprises: collecting starch milk separated by centrifugation, adding water to adjust the concentration of the starch milk to fit, washing the starch milk and collecting bottom starch milk.
    [5]
    The method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 4, wherein the concentration of the adjusted starch slurry is 8 to 12 Bé; the concentration of the starch milk is checked at 18 to 20 Bé; and the concentration of soluble solids in the starch slurry is 0.15-0.5%.
    [6]
    A method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to any preceding claim, wherein the saccharification process step further comprises double-enzymatic saccharification and decolorization of the starch sugar solution
    [7]
    The method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 6, wherein the decolorization process step is a conventional industrial saccharification decolorization.
    [8]
    A method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 7, wherein activated carbon is used for decolorization.
    [9]
    A method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to any preceding claim, wherein the method step of erythritol fermentation comprises: collecting the starch sugar solution as raw material, adding excipients and nutrient salt to prepare an erythritol fermentation medium, controlling the initial glucose concentration at 260 to 300 g/L, inoculate strains for erythritol fermentation after the medium is sterilized, and terminate the fermentation when the glucose concentration is reduced to 0.1 g/L.
    [10]
    A process for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 9, wherein the strains are Yarrowia lipolvtica.
    [11]
    A method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to any one of the preceding claims, further comprising extraction purification of the erythritol.
    [12]
    The method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to claim 11, wherein the extraction and purification method comprises: filtering the collected erythritol fermentation broth to remove yeast, collecting the filtered liquid, and performing concentration, crystallization, dissolution, decolorization, recrystallization, centrifugal separation and drying on the filtered clear liquid to obtain an erythritol product.
    [13]
    Erythritol and/or wheat gluten prepared according to the method for preparing erythritol from wheat starch separated from the wheat gluten production line, according to any one of the preceding claims.
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    同族专利:
    公开号 | 公开日
    CN111454996B|2021-12-07|
    CN111454996A|2020-07-28|
    NL2028015B1|2021-11-09|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    CN1398976A|2001-07-26|2003-02-26|河南莲花味精股份有限公司|Production process of gourmet powder and other amino acids with wheat as raw material|
    CN101265486B|2008-04-29|2011-07-27|安徽瑞福祥食品有限公司|Method for producing biological butanol by fermenting wheat starch emulsion|
    CN101979616B|2010-11-12|2012-04-18|合肥工业大学|Method for producing erythritol by using broken rice|
    CN102154383B|2010-12-29|2011-11-30|保龄宝生物股份有限公司|Method for producing phycite by using corn meal|
    MX350451B|2011-12-22|2017-09-07|Xyleco Inc|Processing biomass for use in fuel cells.|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    CN202010311309.8A|CN111454996B|2020-04-20|2020-04-20|Process method for producing erythritol by producing wheat gluten and co-producing wheat starch|
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